Just 10 norovirus particles (depicted here) are enough to cause infection.
Norovirus is a very contagious virus responsible for around 19–21 million yearly cases of acute gastroenteritis in the United States.
In fact, norovirus is “the leading cause of foodborne illness” in the U.S.
Norovirus is also responsible for up to 1.9 million hospital visits and another 400,000 visits to the emergency department. This costs the U.S. around $2 billion in healthcare expenses and loss of job productivity.
The virus can be contagious at very low levels, with just 10 virus particles being enough to cause an infection.
So, researchers from the University of Arizona (UA) in Tucson set out to create an efficient way to detect even the smallest levels of the virus.
Three researchers co-led the project: Jeong-Yeol Yoon, from the Department of Biomedical Engineering at UA; Soo Chung, a doctoral researcher in Yoon’s laboratory; and Kelly A. Reynolds, chair of the Department of Community, Environment, and Policy in the Mel & Enid Zuckerman College of Public Health at UA.
Yoon presented the research at the American Chemical Society Fall 2019 National Meeting & Exposition in San Diego, CA, and the paper now appears in the journalACS Omega.
How the cheap and quick device works
The virus can spread very quickly through water. Existing devices for detecting norovirus require a laboratory and a range of microscopes, lasers, and costly spectrometers. These are instruments that measure types of radiation and wavelength.
With the new method, the researchers used simple materials including paper and a smartphone. Chung explains how it is possible for paper to transform into microfluidic chips.
“Paper substrate is very cheap and easy to store, and we can fabricate these chips easily,” he says. “The fibrous structure of paper also allows liquid to flow spontaneously without using the pumping systems other chips, such as silicon chips, usually require.”
Typically, scientists measure how light reflects in a sample using spectrometry analysis. However, the nonhomogeneous (porous) aspect of paper, together with its optical opacity, can generate “background scatter and reflection.”
To overcome this obstacle, the researchers developed a method wherein they counted fluorescent beads instead of measuring light intensity.
The method consists of adding water to one end of a paper microfluidic chip and beads made of fluorescent polystyrene to the other end.
Each of these tiny beads is attached to an antibody that fights the virus. If norovirus is present in the water, several antibodies go on to attack it, attaching themselves to the virus particle and creating a fluorescent clump.
“Norovirus particles are too small to be imaged by a smartphone microscope, and so are antibodies,” Yoon explains. “But when you have two or three or more of these beads joined together, that indicates that the norovirus is there, causing the beads to aggregate.”
The clumps of beads are large enough for a smartphone to pick them up and photograph them. The researchers also created a smartphone app that counts the norovirus particles in the sample.
“You don’t have to be a scientist or an engineer to run the device,” says Yoon. “Analysis will be done automatically by the smartphone app, so all you have to worry about is loading a sample of water onto the chip.”
The device is also cheap, with its most expensive component costing less than $50.
“Advances in rapid monitoring of human viruses in water are essential for protecting public health,” says Reynolds.
“This rapid, low cost water quality monitoring technology could be a transformational tool for reducing both local and global disease burdens.”
Kelly A. Reynolds